An assessment of the ability of bacteria to develop resistance to new agents is an important early consideration in the discovery phase. Micromyx utilizes the following assays to provide resistance development data: |
Spontaneous mutation frequency evaluation of frequency with which mutants emerge to an agent in a single step when exposed to suprainhibitory concentrations (4X, 8X the MIC) Serial transfer/passage propensity for mutants to emerge after repeated exposure to sub-inhibitory concentrations of the agent, as measured by increasing MICs |
Spiral gradient plating Gradient plates are prepared that contain subtle changes in the antibiotic concentration throughout the agar. This provides a greater opportunity to select for resistance development. |
Micromyx WMed Innovation Center 4717 Campus Drive Center Kalamazoo, MI 49008 |
Phone: 269-372-3758
Fax: 269-353-5567 |